This readme file was generated on [2023-10-16] by [Mingyue Yuan]


GENERAL INFORMATION

Title of Dataset: Data for soil properties, microbial respiration, microbial growth, for determining resource limitation for microbial growth in climate change simulation treatment in the Subarctic

CREATORS:
Name: Mingyue Yuan
ORCID: 0000-0002-8744-7133
Institution: Department of Biology, Lund University
Email: mingyue.yuan@biol.lu.se

Name: Meng Na
ORCID: 0000-0002-0223-181X
Institution 1 : Department of Biology, Lund University, Ecology Building, 223 62, Lund, Skåne, Sweden.
Institution 2 : School of Forestry, Northeast Forestry University, 150040, Harbin, Heilongjiang, China.
Current institution:Ecology and Environment Department, Anhui Normal University, 241002, Wuhu, Anhui, China.
Email:nameng1991@outlook.com

Name: Lettice Hicks
ORCID: 0000-0002-0265-3887
Institution: Department of Biology, Lund University
Email: Lettice.hicks@biol.lu.se

Name: Johannes Rousk
ORCID: 0000-0002-4985-7262
Institution: Department of Biology, Lund University
Email: johannes.rousk@biol.lu.se

General information


Date of data collection: 
<2019> <2020> 

Geographic location of data collection: 
Abisko, Sweden (68°19'N, 18°50'E) Where the soils were sampled
Lund, Sweden (55°42'N, 13°11'E) Where the laboratory work were carried. 

Information about funding sources that supported the collection of the data: 
This study was supported by grants from The Swedish Research Council Vetenskapsrådet (2020-03858, 2020-04083) and The Knut and Alice Wallenberg Foundation (KAW 2022.0175). 


SHARING/ACCESS INFORMATION

Licenses/restrictions placed on the data: CC-BY licence


DATA & FILE OVERVIEW

File List: 
Microbial biomass response to field treatment
Microbial growth rate response to LFA addition
Normalised growth rate responses to LFA addition
Soil property

Are there multiple versions of the dataset?
If yes, name of file(s) that was updated: 
Why was the file updated? 
When was the file updated? 


LIST OF FILES:
	DOCUMENTATION:
		- Field and laboratory.pdf	(Short description of experiments and sites)
		- README.txt		(This text file)


	DATA IN MACHINE READABLE FORMAT
		- Microbial biomass response to field treatment.csv	(Microbial biomass in different field treatments, data were collected from soils sampled in 2019)
		- Microbial growth rate response to LFA addition.csv	(Microbial growth in response to additions of limiting factor assay, data were collected from soils sampled in 2020)
		- Normalised microbial rate response to LFA addition.csv (Normalised microbial growth in response to additions of limiting factor assay,data were collected from soils sampled in 2020)
		- Soil property.csv	(Including water content, soil organic matter, pH and electrical conductivity, data were collected from soils sampled in 2020)
	


COLUMN HEADINGS FOR MACHINE READABLE TABULAR DATA:
File: Microbial biomass response to field treatment.csv
5 variables: Field treatment, Field replicates, Fungal PLFAs (μg C/g SOM),Bacterial PLFAs (μg C/g SOM),Total PLFAs (μg C/g SOM)
Note:The fungal PLFAs were missing in replicate 6 of field control site, replicate 4 and 6 of chronic litter site, replicate 3 of extreme N site, and replicate 6 of extreme litter site. The Bacterial PLFAs were missing in replicate 6 of field control site, replicate 4 and 6 of chronic litter site, replicate 3 of extreme N site, and replicate 6 of extreme litter site. The total PLFAs were missing in replicate 6 of field control site, replicate 4 and 6 of chronic litter site, replicate 3 and 6 of extreme N site, and replicate 3 and 6 of extreme litter site.

File: Microbial biomass response to field treatment.csv
5 variables: Field treatment, Field replicates, Fungal PLFAs (μg C/g SOM),Bacterial PLFAs (μg C/g SOM),Total PLFAs (μg C/g SOM)

File: Microbial growth rate responses to LFA addition.csv
9 variables: Field treatment, LFA addition, Field replicates, Bacterial growth (ug C/gSOM/hour), Fungal growth (ug C/gSOM/hour), Microbial respiration (ug CO2-C/gSOM/hour),Carbon use efficiency, Leu-to-TdR incorporation ratio

File: Normalised microbial growth rate responses to LFA addition.csv
8 variables: Field treatment, LFA addition, Field replicates, Relative total growth (ug C/gSOM/hour), Relative respiration (ug CO2-C/gSOM/hour), Relative bacterial growth (ug C/gSOM/hour), Relative fungal growth (ug C/gSOM/hour),transformed fungal-to-bacterial ratio (by log10)

File: Soil property.csv
6 variables: Field treatment, Field replicate, Water content (g H2O g-1 dwt),pH,Electrical conductivity (μS cm-1)

VARIABLE LIST (more details please see Field and Laboratory.pdf):
- Field treatment
- LFA addition 
- Bacterial growth (ug C/gSOM/hour)
- Fungal growth (ug C/gSOM/hour)
- Microbial respiration (ug CO2-C/gSOM/hour)
- Carbon use efficiency 
- Relative total growth (ug C/gSOM/hour)
- Relative respiration (ug CO2-C/gSOM/hour) 
- Relative bacterial growth (ug C/gSOM/hour)
- Relative fungal growth (ug C/gSOM/hour)
- Transformed fungal-to-bacterial ratio 
- Water content (g H2O g-1 dwt)
- Soil organic matter
- Electrical conductivity (μS cm-1)
- Fungal PLFAs (μg C/g SOM)
- Bacterial PLFAs (μg C/g SOM)
- Total PLFAs (μg C/g SOM)

NOTE: There are some values missing (marked as #N/A) because there were some technical malfunctions happening during the measurement in the laboratory. For example, some vials for measuring microbial biomass were broken during centrifuging. 

Specialized formats or other abbreviations used: 
LFA:limiting factor assay (limiting factors for microbial growth was determined by microbial growth rate responses to a factorial addition of C,N and P).
PLFA: phospholipid-derived fatty acids (indicator for microbial biomass)
Leu:leucine
TdR:thymidine
SOM:soil organic matter

