CYTOTOXICITY

TREATMENTS:
Poeciliopsis lucida hepatocellular carcinoma cell line (PLHC-1) was exposed to microcystin-LR (MCLR, 0.01, 1 µM), benzo[a]pyrene (BaP, 0.01, 0.1, 1 µM), or mixtures of both chemicals for 24 hours. The control treatment consisted of dimethyl sulfoxide (DMSO, 0.2%), and the positive control was performed with 4,5-dichloro-2-octylisothiazolone (DCOIT) 10 µM (specific Pgp inhibitor). The assays were conducted in 96-well plates, with a cell concentration of 5 × 10⁵ cells/mL in 200 µL solution per well.

Each treatment was performed with 3–8 technical replicates in the 48-well plate (replicates are labeled with letters and numbers in column A of the CSV file). Treatments were carried out on different dates, representing experimental replicates. Due to variations in replicate numbers, certain cells in the CSV file are empty.

CSV File Format: Rows represent technical replicates per treatment, while columns indicate experimental replicates, representing different experimental days for each exposure time.

The technical replicate labels for each type of measurement (e.g., A1 in the EROD_slope file) correspond to the same technical replicate in the file "EROD_protein" but do not correspond across different assays (e.g., A1 in "Rh123_FU" represents a different sample).

FILES (.CSV):
A)Cytotoxicity_FU

MEASUREMENT SPECIFICATIONS
AB: Alamar Blue 
CFDA-AM: 5-Carboxyfluorescein Diacetate -Acetoxymethyl Ester 	
	
Fluorometer plate reader	VICTOR™ 
Name of the label	AB
Number of wells in the plate	8x12
Measurement repeats/well  	1
Delay between repeats (sec)	
Label technology 	Prompt fluorometry
CW-lamp filter name 	F530
CW-lamp filter slot 	A4
Emission filter name 	F590
Emission filter slot 	A8
Measurement time 	0.2 s
Emission aperture 	Normal
CW-lamp energy	15000
	
Fluorometer plate reader	VICTOR™ 
Name of the label	CFDA-AM
Number of wells in the plate	8x12
Measurement repeats/well  	1
Delay between repeats (sec)	
Label technology 	Prompt fluorometry
CW-lamp filter name 	F485
CW-lamp filter slot 	A5
Emission filter name 	F535
Emission filter slot 	A5
Measurement time 	0.2 s
Emission aperture 	Normal
CW-lamp energy	15000

UNITS:
FU (Fluorescent Units)



