EROD 

TREATMENTS: 
Poeciliopsis lucida hepatocellular carcinoma cell line (PLHC-1) was exposed to microcystin-LR (MCLR, 0.01, 1 µM), benzo[a]pyrene (BaP, 0.01, 0.1, 1 µM), or mixtures of both chemicals for 1 to 48 hours. The control treatment consisted of dimethyl sulfoxide (DMSO, 0.2%). The assays were conducted in 48-well plates, with a cell concentration of 6 × 10⁵ cells/mL in 500 µL solution per well.
Each treatment was performed with 3–4 technical replicates in the 48-well plate (replicates are labeled with letters and numbers in column A of the CSV file). Treatments were carried out on different dates, representing experimental replicates. Due to variations in replicate numbers (3 in some cases and 4 in others), certain cells in the CSV file are empty.

There are two sets of experiments:
1) PLHC-1 cells exposure to BaP, MCLR or their mixture for 1 - 48 h.	
2) PLHC-1 cells pre-exposure to BNF (β-Naphthoflavone) for 24 hours, followed by exposure to MCLR or BaP during EROD measurement.

CSV File Format: Rows represent technical replicates per treatment, while columns indicate experimental replicates, representing different experimental days for each exposure time. 

The technical replicate labels for each type of measurement (e.g., A1 in the EROD_slope file) correspond to the same technical replicate in the file "EROD_protein" but do not correspond across different assays (e.g., A1 in "Rh123_FU" represents a different sample).

For the BSA (bovine serum albumin) and the resorufin standard curves we performed duplicates of each concentration (indicated with a label in the column A of the CSV file).

FILES (.CSV):
A)EROD slopes
B)EROD proteins
C)EROD Specific activity
D)EROD BNF-preteatment
E)EROD BSA and Resorufin curves

MEASUREMENT SPECIFICATIONS	
Fluorometer plate reader: VICTOR™ 
Number of wells in the plate: 8x6
Measurement repeats/well: 10
Delay between repeats (sec): 60
Label technology: Prompt fluorometry
CW-lamp filter name: F530
CW-lamp filter slot: A4
Emission filter name: F590
Emission filter slot: A8
Measurement time: 0.1 s
Emission aperture: Normal
CW-lamp energy: 14449

EQUATIONS: 
EROD activity (pmol/min/protein) = EROD slope * resorufin slope-1 * protein concentration-1

UNITS:
Specific EROD-activity: pmol/min/prot
Protein concentrations: mg protein/well


