TREATMENTS:
Poeciliopsis lucida hepatocellular carcinoma cell line (PLHC-1) was exposed to microcystin-LR (MCLR, 0.01, 1 µM), benzo[a]pyrene (BaP, 0.01, 0.1, 1 µM), or mixtures of both chemicals for 90 minutes to 48 hours. The control treatment consisted of dimethyl sulfoxide (DMSO, 0.2%), and the positive control was performed with PSC8333 0.1 µM (specific Pgp inhibitor). The assays were conducted in 48-well plates, with a cell concentration of 6 × 10⁵ cells/mL in 500 µL solution per well.

Each treatment was performed with 3–4 technical replicates in the 48-well plate (replicates are labeled with letters and numbers in column A of the CSV file). Treatments were carried out on different dates, representing experimental replicates. Due to variations in replicate numbers (3 in some cases and 4 in others), certain cells in the CSV file are empty.

CSV File Format: Rows represent technical replicates per treatment, while columns indicate experimental replicates, representing different experimental days for each exposure time.

The technical replicate labels for each type of measurement (e.g., A1 in the EROD_slope file) correspond to the same technical replicate in the file "EROD_protein" but do not correspond across different assays (e.g., A1 in "Rh123_FU" represents a different sample).

For the BSA (bovine serum albumin) standard curve we performed duplicates of each concentration (indicated with a letter and number in the column A of the CSV file).

FILES (.CSV):
A)Rh123 FU (fluorescent units)
B)Rh123 proteins (mg protein/well)
C)Rh23 BSA standard curves 

MEASUREMENT SPECIFICATIONS
Fluorometer plate reader	VICTOR™ 
Number of wells in the plate	8x6
Measurement repeats/well  	1
Delay between repeats (sec)	
Label technology 	Prompt fluorometry
CW-lamp filter name 	F485
CW-lamp filter slot 	A5
Emission filter name 	F535
Emission filter slot 	A5
Measurement time 	0.1 s
Emission aperture 	Normal
CW-lamp energy	20125

EQUATIONS: 
FU/protein concentration

UNITS:
Protein concentrations: mg/well
Rh123 accumulation: FU/mg protein
