TREATMENTS:
Poeciliopsis lucida hepatocellular carcinoma cell line (PLHC-1) was exposed to microcystin-LR (MCLR, 0.01, 1 µM), benzo[a]pyrene (BaP, 0.01, 0.1, 1 µM), or mixtures of both chemicals for 90 minutes to 48 hours. The control treatment consisted of dimethyl sulfoxide (DMSO, 0.2%). The assays were conducted in 6-well plates, with a cell concentration of 2 × 10⁶ cells/mL in 2000 µL solution per well.

Each treatment was performed with 3 technical replicates in the qPCR plate (replicates are labeled with letters and numbers in row 3 of the CSV file). The experimental replicates are indicated in column A of the CSV file. Due to variations in replicate numbers (3 in some cases and 4 in others), certain cells in the CSV file are empty.

CSV File Format: Rows represent experimental replicates per treatment, while columns indicate technical replicates.

The technical replicate labels for each type of measurement (e.g., A1 in the EROD_slope file) correspond to the same technical replicate in the file "EROD_protein" but do not correspond across different assays (e.g., A1 in "Rh123_FU" represents a different sample).

FILES (.CSV):
A)qPCR_Ct_data (cycle treshold)

MEASUREMENT SPECIFICATIONS
SsoAdvanced™ Universal SYBR® Green supermix kit from Bio-Rad, with 2.5 ng/µL cDNA and 400 nM primers final concentration per well, using a MyIQ instrument (BioRad, USA). 
