The data repository is connected to a study that investigates the impact of X-ray treatment on primary ovarian cells (POCs) derived from cortex (cPOCs) and medulla (mPOCs) during in vitro culture. In total 50 samples collected from 5 patients were sequenced and 5 groups (i.e.,control_t1, control_t24, irradiated_t1, irradiated_t4, irradiated_t24) were included in the study.

RNA extraction was performed using the RNeasy Micro Kit (Qiagen, Germany) following the manufacturer’s instructions. Libraries were prepared using the Illumina Stranded mRNA Prep Ligation protocol (Illumina, USA) according to the manufacturer’s instruction, with 10 ng of RNA used as input. 

The dataset contains 100 fastq files from paired-end RNA sequencing. The metadata is described in metadata.xlsx file. A md5sum file is also provided for validation of data integrity. 

The metadata file contains the following information:
Name: code of the fastq files
Patient_ID: patient number
cell type: type of cells (primary) and organ source (ovary)
cell name: cells derived from different ovarian areas, cortex (cPOCs) and medulla (mPOCs)
treatment: control or irradiated
time: recovery time (1, 4, 24 h) after irradiation
Fastq Read 1: name of the Read 1 fastq file 
Fastq Read 2: name of the Read 2 fastq file 

Fastq files were trimmed using TrimGalore (version 0.6.1) to remove the low-quality reads before mapping. STAR aligner (version 2.7.11a) was used to map the trimmed fastq files to human genome hg38. The bam files were aligned using featureCount function in subread package (version 2.0.3) and annotated using UCSC gtf file.