README
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Dataset title:
Data for: Single-cell study of human ovarian response to phthalate exposure reveals glial cell susceptibility and disruption of adhesion and mitochondrial pathways

Recommended Citation:  
Rooda, I., & Damdimopoulou, P. (2026). Data for: Single-cell study of human ovarian response to phthalate exposure reveals glial cell susceptibility and disruption of adhesion and mitochondrial pathways (Version 1) [Data set]. Karolinska Institutet. https://doi.org/10.48723/3vtm-jm80
See the Researchdata.se catalogue entry for this dataset - accessible via the DOI link above - for compete metadata, including the dataset description, contact information and information about how to obtain the dataset. 



Responsible organization:
Karolinska Institutet

Description: Ovarian tissue explants from one individual were exposed to two MEHP concentrations an epidemiologically relevant 20.05 nM and a 1000-fold higher 20.05 µM or a DMSO control. After six days, single-cell RNA sequencing was performed on the cultured tissue samples using the 10x Genomics single-cell pipeline.

License: CC-BY 4.0. This license applies to all transcriptomic data files included in this dataset

Access restrictions: This dataset contains personal data and is subject to legal protections. This dataset can be requested from the Researchdata.se research data catalog using the link above.

Essential information for dataset reuse.
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Ovarian cortex tissue was exposed for six days to two MEHP concentrations an epidemiologically relevant 20.05 nM and a 1000-fold higher 20.05 µM, with complete media changes and re-exposure every 48 hours. A vehicle control (0·1% DMSO) was included. On day six, explants from one patient (donor 1, 23 years old) were dissociated into single cells for library preparation. Cultured ovarian tissue was dissociated to single cells using a combination of enzymatic and mechanic dissociation.  Following dissociation, cells were counted, and viability was assessed to ensure that a high proportion of viable cells (>90% for all samples) proceeded to downstream processing. Three single-cell RNA-sequencing libraries (DMSO, 1X MEHP, 1000X MEHP) were prepared using Chromium Next GEM Single Cell 3’ Reagent Kits v3·1 (10x genomics) according to the manufacturer’s instructions.

Dataset organization
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Datafile descriptions
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The dataset consists of 18 files in fastq format, compressed with GNUzip (gzip), one file in sha256 format and one file in xlsx format. The total size of the dataset is approximately 69 GB.

Reference information
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Abbreviations:
Mono-(2-ethylhexyl) phthalate (MEHP), Dimethyl sulfoxide (DMSO)

Data sources:
NA

Version control:
NA

Source code information:
Single Cell 3’ Reagent Kits v3·1, NextSeq2000, cellranger (version 6·1·1)

Data Quality:
Single cells were counted, and viability was assessed to ensure that a high proportion of viable cells (>90% for all samples)

