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        <identifier>oai:researchdata.se:2022-252-1/2</identifier>
        <datestamp>2023-11-16</datestamp>
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        <setSpec>subject:ssif:106</setSpec>
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        <setSpec>principal:slug:swedish-university-of-agricultural-sciences</setSpec>
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        <oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
          <dc:type>info:eu-repo/semantics/other</dc:type>
          <dc:type>http://purl.org/dc/dcmitype/Dataset</dc:type>
          <dc:identifier>https://doi.org/10.5878/17te-jg54</dc:identifier>
          <dc:title xml:lang="en">Dataset of confocal microscopy from plant samples - High-throughput characterization of cortical microtubule arrays response to anisotropic tensile stress</dc:title>
          <dc:title xml:lang="sv">Datauppsättning från konfokalmikroskopi av växtprover – ”High throughput”-karakterisering av kortikala mikrotubuli-nätverks svar på mekanisk stress.</dc:title>
          <dc:creator>https://orcid.org/0000-0002-8386-7763</dc:creator>
          <dc:creator>https://orcid.org/0000-0003-3643-3978</dc:creator>
          <dc:subject xml:lang="en">Cell Biology</dc:subject>
          <dc:subject xml:lang="sv">Cellbiologi</dc:subject>
          <dc:subject xml:lang="en">Developmental Biology</dc:subject>
          <dc:subject xml:lang="sv">Utvecklingsbiologi</dc:subject>
          <dc:subject xml:lang="en">Plant Biotechnology</dc:subject>
          <dc:subject xml:lang="sv">Växtbioteknologi</dc:subject>
          <dc:description xml:lang="en">The data set contains Tiff Z-stacks from light-grown hypocotyls and cotyledons of cortical microtubule (CMT) reporter lines either with few or no ablated cells from a time series experiment. This data set was analyzed with a new semi-automated image analysis workflow we have developed to quantify CMTs reorganization in individual cells following an ablation (https://github.com/VergerLab/MT_Angle2Ablation_Workflow).

The dataset in the zip file was analyzed using the scripts on GitHub (https://github.com/VergerLab/MT_Angle2Ablation_Workflow). A step by step describes and explains all the scripts of the image analysis procedure. The intermediate data generated by the analysis method can be found on zenodo (https://doi.org/10.5281/zenodo.7436075). 

The documentation file Example_2D_Image.tif gives a visual representation from a typical z-stack.</dc:description>
          <dc:description xml:lang="sv">Datamängden innehåller Tiff Z-stackar från transgena ljusodlade hypokotyler och hjärtblad från Arabidopsis thaliana uttryckande kortikala mikrotubuli (CMT)-reporterlinjer antingen med få eller inga dödade celler från ett tidsserieexperiment. Denna datauppsättning analyserades med ett nytt halvautomatiserat arbetsflöde för bildanalys som vi har utvecklat för att kvantifiera CMT-omorganisation i enskilda celler efter en ablation. (https://github.com/VergerLab/MT_Angle2Ablation_Workflow).

Datamängden i zip-filen analyserades med hjälp av skripten på GitHub (https://github.com/VergerLab/MT_Angle2Ablation_Workflow). Ett steg för steg beskriver och förklarar alla skript för bildanalysproceduren. De mellanliggande data som genereras av analysmetoden kan hittas på zenodo (https://doi.org/10.5281/zenodo.7436075).

Dokumentationsfilen Example_2D_Image.tif ger en visuell representation från en typisk z-stack.</dc:description>
          <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
          <dc:rights>https://creativecommons.org/licenses/by/4.0/</dc:rights>
          <dc:publisher xml:lang="en">Swedish University of Agricultural Sciences</dc:publisher>
          <dc:publisher xml:lang="sv">Sveriges lantbruksuniversitet</dc:publisher>
          <dc:date>2023-05-10T10:49:17.488981Z</dc:date>
          <dc:language>eng</dc:language>
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