2026-10-13 https://api.researchdata.se/oai-pmh

oai:researchdata.se:2023-118-1/1 2026-03-19 subject:ssif:1 subject:ssif:106 subject:ssif:10604 subject:ssif:10609 subject:ssif:10610 subject:ssif:30202 subject:ssif:30112 subject:ssif:301 subject:ssif:302 subject:ssif:3 principal:slug:karolinska-institutet

info:eu-repo/semantics/other http://purl.org/dc/dcmitype/Dataset https://doi.org/10.48723/f2xa-4005 TMT proteomics of erythroblasts from healthy donors and erythroblasts/ring sideroblasts from MDS-RS patients TMT proteomikanalys av erytroblaster från friska donatorer och erytroblaster/ringsideroblaster från patienter med MDS-RS https://orcid.org/0000-0002-0493-5394 https://orcid.org/0000-0002-7839-3743 Natural Sciences Naturvetenskap Biological Sciences Biologi Cell Biology Cellbiologi Genetics and Genomics Genetik och genomik Bioinformatics and Computational Biology Bioinformatik och beräkningsbiologi Hematology Hematologi Basic Cancer Research Basal cancerforskning This dataset consists of semi-quantitative 10-plex TMT proteomics analysis of FACS-separated bone marrow cells obtained from 3 healthy bone marrow donors and 3 MDS-RS patients. The objective of this data collection was to assess several parameters on how the bone marrow of MDS-RS patients differs from that of healthy donors. The samples included in this analyses were 3 samples of sorted GPA+ erythroblasts pooled from 5 healthy donors (2+2+1) and 3 samples of sorted GPA+ erythroblasts plus 3 samples of purified ring sideroblats from 3 MDS-RS patients. This dataset includes the raw mass spectra, QC and processed results. Processing: In short, FACS-separated samples were snap frozen in liquid nitrogen. Cell pellets were lysed with 4 % SDS lysis buffer and prepared for mass spectrometry using a modified version of the SP3 protein clean up and digestion protocol published by Moggridge S. et al 2018. The following files are included in the dataset: - Processed data and analysis.xlsx (2.63 MB) - Variable_list_Processed_data_and_analysis.xlsx (23 KB) - Readme for raw data .zip file.txt (562 bytes) - Readme for Processed data.xlsx file (1.07 KB) - 13553_STD_EAEHPM1_10_TMT10_HiRIEF_3-10pH_20210504_07.15.zip (7.68 GB) Syftet med denna datainsamling var att bedöma flera parametrar om hur benmärgen hos MDS-RS-patienter skiljer sig från den hos friska donatorer. Denna datauppsättning består av semikvantitativ 10-plex TMT-proteomikanalys av FACS-separerade benmärgsceller erhållna från 3 friska benmärgsdonatorer och 3 MDS-RS-patienter. Se den engelska beskrivningen för mer information. Datasetet består av följande: - Processed data and analysis.xlsx (2.63 MB) - Variable_list_Processed_data_and_analysis.xlsx (23 KB) - Readme for raw data .zip file.txt (562 bytes) - Readme for Processed data.xlsx file (1.07 KB) - 13553_STD_EAEHPM1_10_TMT10_HiRIEF_3-10pH_20210504_07.15.zip (7.68 GB) info:eu-repo/semantics/restrictedAccess https://creativecommons.org/licenses/by-nc-nd/4.0/ Karolinska Institutet Karolinska Institutet 2023-10-19T13:50:17.530755Z eng