2026-10-13 https://api.researchdata.se/oai-pmh

oai:researchdata.se:2023-223/1 2026-03-19 subject:ssif:1 subject:ssif:106 subject:ssif:10604 subject:ssif:10609 subject:ssif:10610 subject:ssif:30202 subject:ssif:30112 subject:ssif:301 subject:ssif:302 subject:ssif:3 principal:slug:karolinska-institutet

info:eu-repo/semantics/other http://purl.org/dc/dcmitype/Dataset https://doi.org/10.48723/d7s9-6336 TARGET-seq genotyped single-cell RNA sequencing of hematopoietic stem cells and megakaryocyte-erythroid progenitor cells from a dual SF3B1-mutant MDS-RS patient and 3 healthy donors TARGET-seq genotyped single-cell RNA sequencing of hematopoietic stem cells and megakaryocyte-erythroid progenitor cells from a dual SF3B1-mutant MDS-RS patient and 3 healthy donors https://orcid.org/0000-0002-0493-5394 https://orcid.org/0000-0002-7839-3743 Affaf Aliouat Sten Eirik Jacobsen Petter Woll Natural Sciences Naturvetenskap Biological Sciences Biologi Cell Biology Cellbiologi Genetics and Genomics Genetik och genomik Bioinformatics and Computational Biology Bioinformatik och beräkningsbiologi Hematology Hematologi Basic Cancer Research Basal cancerforskning This dataset consists of TARGETseq (genotype-targeted plate-based SmartSeq2) single-cell RNA sequencing data of purified hematopoietic stem cells (HSC) and megakaryocyte-erythroid progenitors (MEP) from the bone marrow of a dual SF3B1-mutant MDS-RS patient (Patient 1 in the associated publication) over 2 timepoints (39 months post-diagnosis, with dominant SF3B1-N626D, and 118 months post-diagnosis, with dominant SF3B1-K666N); and purified HSC/MEP from the bone marrow of three healthy donors. The objective of this data collection was to assess the molecular characteristics that increase fitness in SF3B1-mutant HSC as compared to normal HSC. The dataset is approximately 187 GB and includes the file types: zip, xlsx, bam, bam.bai, rds. Denna datauppsättning består av TARGETseq (genotypriktad plattbaserad SmartSeq2) RNA-sekvenseringsdata från renade hematopoetiska stamceller (HSC) och megakaryocyt-erytroida progenitorer (MEP) från benmärgen hos en dubbel SF3B1-mutant MDS-RS patient vid två tillfällen poäng (39 månader efter diagnos och 118 månader efter diagnos); och renad benmärg HSC/MEP från tre friska donatorer. Syftet med denna datainsamling var att bedöma de molekylära egenskaperna som ökar "fitness" i SF3B1-mutant HSC jämfört med normal HSC. Se den engelska beskrivningen för mer information. info:eu-repo/semantics/restrictedAccess https://creativecommons.org/licenses/by-nc-nd/4.0/ Karolinska Institutet Karolinska Institutet 2024-04-18T13:21:58.662934Z eng