2026-10-14 https://api.researchdata.se/oai-pmh

oai:researchdata.se:2025-242/2 2026-03-02 subject:ssif:10401 subject:ssif:10502 subject:ssif:10506 subject:ssif:10604 subject:ssif:104 subject:ssif:105 subject:ssif:106 subject:ssif:1 principal:slug:swedish-university-of-agricultural-sciences

info:eu-repo/semantics/other http://purl.org/dc/dcmitype/Dataset https://doi.org/10.5878/r92f-7869 Subcellular Thiol Functional Group Distribution in Geobacter sulfurreducens Determined by Hg LIII-Edge EXAFS: Research Data Subcellular Thiol Functional Group Distribution in Geobacter sulfurreducens Determined by Hg LIII-Edge EXAFS: Research Data https://orcid.org/0000-0002-8610-0525 Analytical Chemistry Analytisk kemi Environmental Sciences Miljövetenskap Geochemistry Geokemi Cell Biology Cellbiologi This dataset contains the research data underlying the article “Subcellular Thiol Functional Group Distribution in Geobacter sulfurreducens Determined by Hg LIII-Edge EXAFS,” published in Frontiers in Microbiology (https://doi.org/10.3389/fmicb.2025.1728775). The dataset comprises measurements of subcellular biomass, total organic carbon (TOC), total sulfur (TS), thiol groups (RSH), and spectroscopic data (EXAFS and XANES) collected to characterize subcellular compartments of G. sulfurreducens PCA (Caccavo et al., 1994), including extracellular, periplasmic, cytoplasmic, and membrane fractions. TOC and TS were quantified using an elemental analyzer (Vario MACRO cube) with a relative standard deviation (RSD) of 0.5%. Total mercury concentrations were determined by combustion atomic absorption spectrometry with a direct mercury analyzer (DMA-80, Milestone; detection limit = 0.01 ng; RSD = 6%). Sulfur K-edge XANES spectra were collected at the 4B7A Medium Energy X-ray Beamline of the Beijing Synchrotron Radiation Facility. Data processing, including normalization and linear combination fitting (LCF), was performed using the Larch software package. Hg LIII-edge EXAFS spectra were acquired at Diamond Light Source (Beamline I20-scanning). Data analysis and fitting were carried out using WinXAS in combination with FEFF-7. Further details are provided in the accompanying README.txt file. Detta dataset innehåller de forskningsdata som ligger till grund för artikeln ”Subcellular Thiol Functional Group Distribution in Geobacter sulfurreducens Determined by Hg LIII-Edge EXAFS”, publicerad i Frontiers in Microbiology (https://doi.org/10.3389/fmicb.2025.1728775). Datasetet omfattar mätningar av subcellulär biomassa, totalt organiskt kol (TOC), totalt svavel (TS), tiolgrupper (RSH) samt spektroskopiska data (EXAFS och XANES) insamlade för att karakterisera subcellulära fraktioner av G. sulfurreducens PCA (Caccavo et al., 1994), inklusive extracellulära, periplasmiska, cytoplasmatiska och membranfraktioner. TOC och TS kvantifierades med en elementaranalysator (Vario MACRO cube) med en relativ standardavvikelse (RSD) på 0,5 %. Totala kvicksilverkoncentrationer bestämdes med förbränningsatomabsorptionsspektrometri med en direktkvicksilveranalysator (DMA-80, Milestone; detektionsgräns = 0,01 ng; RSD = 6 %). Svavel K-kant XANES-spektra samlades in vid 4B7A Medium Energy X-ray Beamline vid Beijing Synchrotron Radiation Facility. Databearbetning, inklusive normalisering och linjärkombinationsanpassning (LCF), utfördes med Larch-programvaran. Hg LIII-kant EXAFS-spektra erhölls vid Diamond Light Source (Beamline I20-scanning). Dataanalys och anpassning utfördes med WinXAS i kombination med FEFF-7. Ytterligare detaljer finns i den medföljande filen README.txt. info:eu-repo/semantics/openAccess https://creativecommons.org/publicdomain/zero/1.0/ Swedish University of Agricultural Sciences Sveriges lantbruksuniversitet 2026-03-02T07:27:53.054912Z eng