2026-10-11 https://api.researchdata.se/oai-pmh

oai:researchdata.se:doi-10-17044-scilifelab-14315777/0 2024-02-12 subject:ssif:3 subject:ssif:10408 subject:ssif:104 subject:ssif:1 principal:slug:royal-institute-of-technology

info:eu-repo/semantics/other http://purl.org/dc/dcmitype/Dataset https://doi.org/10.17044/SCILIFELAB.14315777 Immunofluorescence data of 700 host cell proteins in Sars-CoV2 infected vs non-infected VeroE6 cells Jayasankar Mohanakrishnan Kaimal Ulrika Axelsson Trang H. Le https://orcid.org/0000-0001-5744-3206 Hao Xu Hanna Axelsson Anna Bäckström Duncan Njenda Elisabeth Moussaud-Lamodière Wei Ouyang Carolina Oses Sepulveda Brinton Seashore-Ludlow Caroline Vernersson Ali Mirazimi Päivi Östling Emma Lundberg Charlotte Stadler Medical and Health Sciences Medicin och hälsovetenskap Biochemistry Biokemi The data set contains ≈ 75,000 confocal images of mixed Vero E6 cell populations (SARS-CoV2 infected vs non-infected). Cells had been infected with the virus for 1 h and fixed 24 hour post infection in 96 well plates. Using immunofluorescence (4 colours) cells were stained for the SARS-CoV2 virus (red), the endoplasmic reticulum (yellow) and nucleus (blue) – same for all images in the data set and used as reference markers. On top of this, 700 different target proteins were stained – one per well (green). Images were acquired with an Opera Phoenix microscope at 63X, using 9 FoV and 3 z-planes per protein target. IN the original study, intensity differences and spatial re-distribution of the target proteins were evaluated in infected vs the non-infected cells. The image resource was generated and described in detail in this research article: (to be added). https://creativecommons.org/licenses/by/4.0/ Royal Institute of Technology Kungliga Tekniska högskolan 2022-02-17T00:00:00Z