2026-10-14 https://api.researchdata.se/oai-pmh

oai:researchdata.se:doi-10-17044-scilifelab-19761343/0 2022-06-21 subject:ssif:3 subject:ssif:30199 subject:ssif:301 subject:ssif:30203 subject:ssif:302 principal:slug:uppsala-university

info:eu-repo/semantics/other http://purl.org/dc/dcmitype/Dataset https://doi.org/10.17044/SCILIFELAB.19761343 Chromium Next GEM Single Cell 3’ RNA-sequencing of tumor cell line co-cultured with PBMC and treated with trifluridine or DMSO control Claes Andersson Tove Selvin Medical and Health Sciences Medicin och hälsovetenskap Other Basic Medicine Andra medicinska och farmaceutiska grundvetenskaper Cancer and Oncology Cancer och onkologi Chromium Next GEM Single Cell 3’ RNA-sequencing.  HCT116 were seeded in 6-well Nunc plates (50,000 cells/3mL/well) and precultured for 24 h before PBMCs were added at a 1:8 ratio. Co-cultures were treated with DMSO vehicle (0.1%) or FTD (3mM) for 12 h or 72 h. MACS Dead Cell Removal Kit (Miltenyi Biotec, Gladbach, DEU) was performed according to the manufacturer’s instructions on cells treated for 72 h to increase the viability of the samples before RNA-sequencing. The viability of the samples treated for 12 h was not subjected to Dead Cell Removal as the viability was already sufficient. All samples were washed in PBS with 0.04% BSA (2x1mL).  Chromium Next GEM Single Cell 3’ library preparation and RNA-sequencing were performed by the SNP&SEQ Technology Platform (National Genomics Infrastructure (NGI), Science for Life Laboratory, Uppsala University, Sweden). info:eu-repo/semantics/restrictedAccess Uppsala University Uppsala universitet 2022-06-21T00:00:00Z