2026-10-14 https://api.researchdata.se/oai-pmh

oai:researchdata.se:doi-10-17044-scilifelab-20107199/0 2022-08-02 subject:ssif:3 subject:ssif:10408 subject:ssif:104 subject:ssif:1 principal:slug:uppsala-university

info:eu-repo/semantics/other http://purl.org/dc/dcmitype/Dataset https://doi.org/10.17044/SCILIFELAB.20107199 Spatiotemporally controlled generation of NTPs for single-molecule studies of NTP-driven processes https://orcid.org/0000-0002-8559-8894 https://orcid.org/0000-0001-5841-0991 https://orcid.org/0000-0002-5263-3577 https://orcid.org/0000-0003-2875-7315 https://orcid.org/0000-0003-0901-4188 https://orcid.org/0000-0001-9446-6730 Mikhail A. Khodorkovskiy Sebastian Deindl Medical and Health Sciences Medicin och hälsovetenskap Biochemistry Biokemi Data supporting the findings of the paper titled "Spatiotemporally controlled generation of NTPs for single-molecule studies" in Nature Chemical Biology which describes a method for controlling NTP-driven reactions in single-molecule experiments via the local generation of NTPs (LAGOON) that increases the measurement throughput and enables single-turnover observations.  The dataset contains single-molecule FRET data capturing T4 helicase DNA unwinding, nucleosome remodeling by Chd1 and the invasion of a nucleosome by the T7 RNA polymerase. Additionally, the dataset contains acoustic force spectroscopy data on E. coli RNA polymerase elongation. https://creativecommons.org/licenses/by/4.0/ Uppsala University Uppsala universitet 2022-08-02T00:00:00Z