2026-10-11
https://api.researchdata.se/oai-pmh
oai:researchdata.se:doi-10-17044-scilifelab-20338869/0
2023-05-30
subject:ssif:3
subject:ssif:106
subject:ssif:1
subject:ssif:10408
subject:ssif:104
principal:slug:karolinska-institutet
info:eu-repo/semantics/other
http://purl.org/dc/dcmitype/Dataset
https://doi.org/10.17044/SCILIFELAB.20338869
High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler
https://orcid.org/0000-0002-4915-388X
Taras Sych
Jan Schlegel
Hanna Barriga
Miina Ojansivu
Leo Hanke
Florian Weber
R. Beklem Bostancioglu
Kariem Ezzat
Herbert Stangl
Birgit Plochberger
Jurga Laurencikiene
Samir El Andaloussi
Daniel Furth
https://orcid.org/0000-0002-7335-266X
Medical and Health Sciences
Medicin och hälsovetenskap
Biological Sciences
Biologi
Biochemistry
Biokemi
This item containst data sets for Sych et al, Nature Biotechology, 2023.
It contains raw fluroescence fluctuation data as excle sheet and raw figure files.
Abstract:
We introduce a method, single-particle profiler (SPP), that provides single-particle information on the content and biophysical properties of thousands of particles in the size range 5-150 nm. We apply SPP to measure the mRNA encapsulation efficiency of lipid nanoparticles, viral binding efficiency of different nanobodies, and biophysical heterogeneity of liposomes, lipoproteins, exosomes and viruses.
Data usage
Researchers are welcome to use the data contained in the dataset for any projects. Please cite this item upon use or when published. We encourage reuse using the same CC BY 4.0 License.
Data Content
FCS files as raw data (.fcs)
Excel and Prism files for graphs
# Software to open files:
.xlsx: Microsoft Excel
.pzfx: GraphPad Prism
.svg: Inkscape (https://inkscape.org/)
.fcs: Single Particle Profiler (https://github.com/taras-sych/Single-particle-profiler)
.ipynb: Jupyter Notebook, installed as part of anaconda platform, python 3.8.8 (https://www.anaconda.com/)
.py: executed via anaconda platform, python 3.8.8 (https://www.anaconda.com/)
https://creativecommons.org/licenses/by/4.0/
Karolinska Institutet
Karolinska Institutet
2023-05-03T00:00:00Z