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        <datestamp>2025-02-04</datestamp>
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          <dc:identifier>https://doi.org/10.17044/SCILIFELAB.28342484</dc:identifier>
          <dc:title xml:lang="en">RNA-Seq data from Ovarian Tumours</dc:title>
          <dc:creator>https://orcid.org/0009-0000-7130-8668</dc:creator>
          <dc:creator>Julia Lindberg Hedlund</dc:creator>
          <dc:creator>Ulf Gyllensten</dc:creator>
          <dc:creator>Karin Sundfeldt</dc:creator>
          <dc:creator>Karin Stålberg</dc:creator>
          <dc:creator>https://orcid.org/0000-0002-5056-9137</dc:creator>
          <dc:subject xml:lang="en">Medical and Health Sciences</dc:subject>
          <dc:subject xml:lang="sv">Medicin och hälsovetenskap</dc:subject>
          <dc:subject xml:lang="en">Genetics and Genomics</dc:subject>
          <dc:subject xml:lang="sv">Genetik och genomik</dc:subject>
          <dc:subject xml:lang="en">Cancer and Oncology</dc:subject>
          <dc:subject xml:lang="sv">Cancer och onkologi</dc:subject>
          <dc:description xml:lang="en">The data presented in this item contain sensitive information that cannot be shared openly. Work on depositing the data in FEGA Sweden has been initiated. FEGA Sweden is a national node of the Federated European Genome-phenome Archive (FEGA), which allows data to be shared under controlled access. The datasets in FEGA Sweden are findable through the European Genome-phenome Archive web portal (https://ega-archive.org).

This dataset refers to the raw data used in the preprint "Deep plasma proteomics identifies and validates an eight-protein biomarker panel that separate benign from malignant tumors in ovarian cancer" available on MedRxiv (DOI: 10.1101/2024.10.10.24315232v1)

Data Set Description

Samples: 81 fresh frozen tumor tissue samples, either benign or malignant, from women with suspected ovarian cancer were used for analysis of mRNA expression.

Library Preparation and Sequencing: Sequencing libraries were prepared from 122-194 ng (three samples), 200 ng (seven samples) or 500 ng (71 samples) total RNA using the TruSeq stranded mRNA library preparation kit (cat# 20020595, Illumina Inc.) including polyA-selection. The libraries were then sequenced on a NovaSeq 6000 system (Illumina Inc.) on S4 flowcells with version 1.5 sequencing chemistry on three lanes. Paired-end sequencing of with read lengths of 150 bp was used.

Library preparation and sequencing was performed by the SNP&amp;SEQ Technology platform, SciLifeLab, National Genomics Infrastructure Uppsala, Sweden.</dc:description>
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          <dc:publisher xml:lang="en">Uppsala University</dc:publisher>
          <dc:publisher xml:lang="sv">Uppsala universitet</dc:publisher>
          <dc:date>2025-02-04T00:00:00Z</dc:date>
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