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        <identifier>oai:researchdata.se:doi-10-17044-scilifelab-29315642/0</identifier>
        <datestamp>2026-01-21</datestamp>
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          <dc:identifier>https://doi.org/10.17044/SCILIFELAB.29315642</dc:identifier>
          <dc:title xml:lang="en">Live-cell Phase Contrast image dataset using a balanced class benchmark assay</dc:title>
          <dc:creator>https://orcid.org/0000-0001-7204-0026</dc:creator>
          <dc:creator>https://orcid.org/0000-0001-8357-5018</dc:creator>
          <dc:creator>https://orcid.org/0000-0002-8083-2864</dc:creator>
          <dc:creator>https://orcid.org/0009-0004-5180-9704</dc:creator>
          <dc:creator>https://orcid.org/0000-0003-3799-6094</dc:creator>
          <dc:creator>https://orcid.org/0000-0002-7671-3707</dc:creator>
          <dc:creator>https://orcid.org/0000-0002-3733-8975</dc:creator>
          <dc:creator>https://orcid.org/0000-0003-3799-6684</dc:creator>
          <dc:creator>https://orcid.org/0000-0002-1898-4453</dc:creator>
          <dc:subject xml:lang="en">Medical and Health Sciences</dc:subject>
          <dc:subject xml:lang="sv">Medicin och hälsovetenskap</dc:subject>
          <dc:description xml:lang="en">Dataset generated for 10.1101/2025.04.22.649936 (https://doi.org/10.1101/2025.04.22.649936) , using an assay with 6 balanced MoA classes. The dataset contains time-lapse images of A549 and U2OS cells.

Paired github repository: https://github.com/edvinforsgren/LCTP

The data contains time-lapse images of two cell lines (A549 and U2OS). The cells were cultivated in an benchmark assay developed for a paper where analysis of a single time-point versus analysis of the full time-lapse was evaluated as a way to determine if adding temporal data could enhance the mined features and improve downstream tasks. 

The experimental set-up consists of three biological replicates (i.e. three plates with unique layout variants) for each cell-line (A549 and U2OS) with 5 technical replicates (i.e. 5 wells) and 15 DMSO control wells each - for a total of 15 replicates and 45 DMSO replicates for each cell-line. 

The time-lapse acquisition was taken with 2 hours between each acquisition over a duration of 72 hours.</dc:description>
          <dc:rights>https://creativecommons.org/licenses/by-nc-sa/4.0/</dc:rights>
          <dc:publisher xml:lang="en">Umeå University</dc:publisher>
          <dc:publisher xml:lang="sv">Umeå universitet</dc:publisher>
          <dc:date>2026-01-21T00:00:00Z</dc:date>
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