2026-10-14 https://api.researchdata.se/oai-pmh

oai:researchdata.se:doi-10-17044-scilifelab-30103798/0 2025-09-16 subject:ssif:3 subject:ssif:10408 subject:ssif:104 subject:ssif:1 subject:ssif:106 subject:ssif:10609 principal:slug:uppsala-university

info:eu-repo/semantics/other http://purl.org/dc/dcmitype/Dataset https://doi.org/10.17044/SCILIFELAB.30103798 Plasmid sequences corresponding to the study "Origin and evolution of key enzymes in the anammox pathway revisited". https://orcid.org/0000-0003-3493-4154 https://orcid.org/0000-0002-0829-9882 Erik Hagström https://orcid.org/0000-0002-9131-4122 https://orcid.org/0000-0001-8354-2398 Siv G. E. Andersson Medical and Health Sciences Medicin och hälsovetenskap Biochemistry Biokemi Biological Sciences Biologi Genetics and Genomics Genetik och genomik pHA-1 plasmid sequences with synthetic gene constructs in which the N-terminal sequences of the hydrazine dehydrogenase (HDH) and the gamma-subunit of the hydrazine synthase (HZS) from “Ca. Kuenenia stuttgartiensis” strain MBR1 were fused to the phoA gene lacking its own native signal sequence. For comparison, the signal sequences of two periplasmic proteins in “Ca. Kuenenia stuttgartiensis” strain MBR1, a flagellar P-ring protein, and an outer membrane lipoprotein-sorting protein, were also fused to the phoA gene. As positive controls, we used plasmids containing the phoA gene with its native signal sequence as well as a fusion of the lepB and the phoA genes. As negative controls, we used a plasmid that contained the phoA gene without a signal sequence. https://creativecommons.org/licenses/by/4.0/ Uppsala University Uppsala universitet 2025-09-16T00:00:00Z