2026-10-14 https://api.researchdata.se/oai-pmh

oai:researchdata.se:doi-10-17045-sthlmuni-7117430/0 2018-12-27 subject:ssif:10408 subject:ssif:104 subject:ssif:1 subject:ssif:30108 subject:ssif:301 subject:ssif:3 subject:ssif:10615 subject:ssif:106 principal:slug:stockholm-university

info:eu-repo/semantics/other http://purl.org/dc/dcmitype/Dataset https://doi.org/10.17045/STHLMUNI.7117430 NrdJm phylogeny https://orcid.org/0000-0002-8779-6464 Christoph Loderer Karin Holmfeldt Biochemistry Biokemi Cell and Molecular Biology Cell- och molekylärbiologi Evolutionary Biology Evolutionsbiologi All NrdJm sequences from NCBI’s RefSeq database were downloaded from RNRdb (http://rnrdb.pfitmap.org) and clustered with USEARCH (Edgar 2010) at 90% sequence identity to reduce redundancy. Non full-length sequences and sequences of dubious quality were manually removed, before aligning all 363 sequences with ProbCons (Do et al. 2005). Trustworthy alignment positions were selected with the BMGE algorithm (Criscuolo and Gribaldo 2010) using the BLOSUM30 substitution matrix, ending up with 363 well-aligned positions forming 350 distinct alignment patterns. RAxML version 8.2.4 (Stamatakis 2014) was used to estimate a phylogeny, using the PROTGAMMAAUTO model, rapid bootstrapping with the autoMRE bootstopping followed by a full maximum likelihood tree search. The file is viewable with Dendroscope (Huson et al. 2007). v2: Addition of Lactobacillus leichmannii sequence. https://creativecommons.org/licenses/by/4.0/ Stockholm University Stockholms universitet 2018-09-21T00:00:00Z